Cates, 69, "died peacefully in her sleep," her daughter Sheri Cates Morgan posted on Facebook.
"Darlene was the best acting mom I ever had the privilege of working alongside," DiCaprio wrote. "Her endearing personality and incredible talent will live on in the memories of those who knew her, and those who loved her work. My thoughts and prayers are with her family during this difficult time."
The movie, in which he played Johnny Depp's developmentally disabled brother, earned him his first Oscar nomination.
The actress made her film debut in the 1993 indie film after "Gilbert Grape" author and screenwriter Peter Hedges saw her on an episode of Sally Jessy Raphael's talk show titled "Too Heavy to Leave Their House."
After the film she appeared in guest roles in "Touched by an Angel," "Picket Fences" and the TV movie "Wolf Girl."
Gilbert Grape (Johnny Depp) is a small-town young man with a lot of responsibility. Chief among his concerns are his mother (Darlene Cates), who is so overweight that she can't leave the house, and his mentally impaired younger brother, Arnie (Leonardo DiCaprio), who has a knack for finding trouble. Settled into a job at a grocery store and an ongoing affair with local woman Betty Carver (Mary Steenburgen), Gilbert finally has his life shaken up by the free-spirited Becky (Juliette Lewis). Read More